David
Haake, MD
Associate Professor of Medicine
David Geffen School of Medicine at UCLA
Division of Infectious Diseases, 111F
VA Greater Los Angeles Healthcare System
11301 Wilshire Blvd
Los Angeles, CA 90073
fax (310)268-4928
email dhaake@ucla.edu
Educational Background:
Harvard
University, Cambridge, MA B.A. 1979 Chemistry
New York University School of Medicine M.D. 1983 Medicine
UCLA School of Medicine, Los Angeles, CA Postdoc. 1991 Spirochetal Pathogenesis
Honors
1989-1991 Burroughs Wellcome Fund Postdoctoral Fellowship, Infectious Diseases
Society of America.
1995 Frontiers of Science Award, UCLA School of Medicine.
Selected peer-reviewed publications (in chronological order).
1. Haake, D. A., Frangione, B., and Franklin, E. C., "The modification of human immunoglobulin binding to Staphylococcal protein A using diethylpyrocarbonate," J. Immunol., 129:190-2 (1982).
2. Thomas, D. D., Navab, M., Haake, D. A., Fogelman, A. M., Miller, J. N., and Lovett, M. A., "Treponema pallidum invades intercellular junctions of endothelial cell monolayers," Proc. Natl. Acad. Sci. USA, 85:3608-12 (1988).
3. Blanco, D. R., Walker, E. M., Haake, D. A., Champion, C. I., Miller, J. N., and Lovett, M. A. Complement activation limits the rate of in vitro treponemicidal activity and correlates with antibody-mediated aggregation of Treponema pallidum rare outer membrane protein," J. Immunol. 144:1914-21 (1990).
4. Haake, D. A., Lovett, M. A., "Interjunctional invasion of endothelial monolayers by Treponema pallidum.." In: Iglewski BH, Clark VL, eds. The Bacteria, Volume XI. The Molecular Basis of Bacterial Pathogenesis. Academic Press, Orlando, FL, pp. 297-315 (1990).
5. Haake, D. A., Walker, E. M., Blanco, D. R., Bolin, C. A., Miller, J. N., and Lovett, M. A., "Changes in the surface of Leptospira interrogans serovar grippotyphosa during in vitro cultivation," Infect. Immun., 59:1131-40 (1991).
6. Blanco, D. R., Giladi, M., Champion, C. I., Haake, D. A., Chikami, G. K., Miller, J. N., and Lovett, M. A., "Identification of Treponema pallidum subspecies pallidum genes encoding signal peptides and membrane spanning sequences using a novel alkaline phosphatase expression vector," Molec. Microbiol., 5:2405-15 (1991).
7. Giladi, M., Champion, C. I., Haake, D. A., Blanco, D. R., Champion, C. I., Miller, J. F., Miller, J. N., and Lovett, M. A., "Use of the 'blue halo' assay in the identification of genes encoding membrane proteins with cleavable signal peptides: cloning of a Borrelia burgdorferi plasmid gene with a signal peptide," J. Bacteriol., 175:4129-4136 (1993).
8. Haake, D. A., Champion, C. I., Martinich, C., Shang, E. S., Blanco, D. R., Miller, J. N., and Lovett, M. A., "Molecular cloning and sequence analysis of the gene encoding OmpL1: a transmembrane outer membrane protein of pathogenic Leptospira spp." J. Bacteriol., 175:4225-4234 (1993).
9. Champion, C. I., Blanco, D. R., Skare, J. T., Haake, D. A., Giladi, M., Foley, D., Miller, J. N., and Lovett, M. A., "A 9.0-kilobase-pair circular plasmid of Borrelia burgdorferi encodes an exported protein: Evidence for expression only during infection," Infect. Immun. 62:2653-2661 (1994).
10. Haake, D. A., and Lovett, M. A., "Interjunctional invasion of endothelial cell monolayers." In: Clark VL, Bavoil PM, eds. Methods in Enzymology. Bacterial Pathogenesis, Part B. Academic Press, Orlando, FL, Vol. 236, pp. 447-463 (1994).
11. Shang, E. S., Exner, M. M., Summers, T. A., Martinich, C., Champion, C. I., Hancock, R. E. W., and Haake, D. A., "The rare outer membrane protein, OmpL1, of pathogenic Leptospira species is a heat-modifiable porin," Infect. Immun. 63:3174-3181 (1995).
12. Shang, E. S., Summers, T. A., and Haake, D.A. "Molecular cloning and sequence analysis of the gene encoding LipL41, a surface-exposed lipoprotein of pathogenic Leptospira species" Infect. Immun. 64:2322-2330 (1996).
13. Haake, D. A., and Lovett, M. A., "Interjunctional invasion of endothelial cell monolayers." In: Clark VL, Bavoil PM, eds. Bacterial Pathogenesis, Academic Press, Orlando, FL, pp. 711-727 (1997).
14. Haake, D. A., Martinich, C., Summers, T. A., Shang, E. S., Pruetz, J. D., McCoy, A. M., Mazel, M. K., and Bolin, C. A. "Characterization of leptospiral outer membrane lipoprotein LipL36: Downregulation associated with late-log-phase growth and mammalian infection" Infect. Immun. 66:1579-1587 (1998).
15. Barnett, J. K., Barnett, D., Bolin, C. A., Summers, T. A., Wagar, E. A., Cheville, N., Hartskeerl, R., and Haake, D. A. "Expression and distribution of leptospiral outer membrane components during renal infection in hamsters" Infect. Immun. 67:853-861 (1999).
16. Haake, D. A., Mazel, M. K., McCoy, A. M., Milward, F., Chao, G., Matsunaga, J., and Wagar, E. A., "Leptospiral outer membrane proteins OmpL1 and LipL41 exhibit synergistic immunoprotection," Infect. Immun. 67:6572-6582 (1999).
17. Haake, D. A., Chao, G., Zuerner, R. L., Barnett, J. K., Barnett, D., Mazel, M., Matsunaga, J., Levett, P. N., and Bolin, C. A., "The leptospiral major outer membrane protein, LipL32, is a lipoprotein expressed during mammalian infection," Infect. Immun. 68:2276-2285 (2000).
18. Haake, D. A., "Spirochetal lipoproteins and pathogenesis," Microbiology 146:1491-1504 (2000).
19. Zuerner, R., Haake, D., Adler, B., and Segers, R., "Technological advances in the molecular biology of Leptospira," J. Mol. Microbiol. Biotechnol. 2:455-462 (2000).
20. Werts, C., Tapping, R. I., Mathison, J. C., Chuang, T.-H., Kravchenko, V., Saint Girons, I., Haake, D., Godowski, P. J., Hayashi, F., Ozinsky, A., Underhill, D., Aderem, A., Tobias, P. S., and Ulevitch, R. J., "Leptospiral endotoxin activates cells via a TLR2-dependent mechanism," Nature Immunology 2:346-352 (2001).
21. Zuerner, R., Haake, D., Adler, B., and Segers, R., "Technological advances in the molecular biology of Leptospira," in "The Spirochetes: Molecular and Cellular Biology", Horizon Scientific Press, Wymondham, UK (2001).
22. Guerreiro, H., Croda, J., Flannery, B., Mazel, M., Matsunaga, J., Reis, M. G., Levett, P. N., Ko, A. I., and Haake, D. A., "Leptospiral proteins recognized by the humoral immune response during human leptospirosis," Infect. Immun. 69:4958-4968 (2001).
23. Flannery, B., Costa, D., Carvalho, F. P., Guerreiro, H., Matsunaga, J., Da Silva, E. D., Ferreira, A. G. P., Riley, L. W., Dos Reis, M.G., Haake, D. A., and Ko, A. I., "Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis," J. Clin. Microbiol. 39:3303-3310 (2001).
24. Matsunaga, J., Young, T. A., Barnett, J. K., Barnett, D., Bolin, C. A., and Haake, D. A. "A novel 45-kilodalton leptospiral protein that is processed to a 31-kilodalton growth phase regulated periphernal membrane protein," Infect. Immun. 70:323-34 (2002).
25. Wild, C. J., Greenlee, J. J., Bolin, C. A., Barnett, J. K., Haake, D. A., and Cheville, N. F. "An improved immunohistochemical diagnostic technique for canine leptospirosis using anti-leptospiral antibodies on renal tissue." J. Vet. Diagn. Invest. 14:20-24 (2002).
26. Haake, D. A., Dundoo, M., Cader, R., Kubak, B. M., Hartskeerl, R. A., Sejvar, S., and Ashford, D. A. "Leptospirosis, water sports, and chemoprophylaxis," Clin. Infect. Dis. 34:E40-43 (2002).
27. Cullen, P. A., Cordwell, S. J., Bulach, D. M., Haake, D. A., and Adler, B. "Global analysis of outer membrane proteins from Leptospira interrogans serovar Lai," Infect. Immun. 70:2311-2318 (2002).
28. Haake, D. A., and Matsunaga, J., "Characterization of the leptospiral outer membrane and description of three novel leptospiral membrane proteins," Infect. Immun. 70:4936-45 (2002).
Ongoing Research Support
2 R01 AI34431-6 Haake (PI) 12/01/02 - 11/30/06
NIH/NIAID
Outer Membrane Proteins of Pathogenic Leptospira Species.
This study involves identification of novel outer membrane proteins involved in leptospiral pathogenesis and immunity.Merit Award Haake (PI) 4/01/02 - 3/31/05
Veterans Affairs Medical Research Fund
Mechanisms of Outer Membrane Protein Mediated Immunoprotection.
This study involves examination of immunoprotection using two leptospiral outer membrane proteins: OmpL1 and LipL41.Collaborative Grant Haake / de la Pena-Moctezuma (coPIs) 7/01/02 - 12/31/03
UC MEXUS-CONACYT
Leptospiral outer membrane proteins (OMP) and its role in pathogenesis and immunity
This study will identify the cultural and environmental conditions affecting expression of selected OMPs, use in vitro adhesion and opsonophagocytic assays to examine their role in pathogenesis, clone and express the OMPs in E. coli and undertake immunoprotection studies with purified recombinant proteins.Merit Award Matsunaga (PI) 10/01/02 - 09/30/06
Veterans Affairs Medical Research Fund
Virulence-Associated Proteins of Pathogenic Leptospira Species
The major goals of this project are to identify virulence-associated leptospiral proteins, characterize their role(s) in pathogenesis, and evaluate their immunoprotective potential.
Role: Collaborator1 R01 EB00127-01 Churchill (PI) 7/01/02 - 6/30/07
NIH/NIBIB Bioengineering Research Partnership Grant
Uropathogen Detection Using DNA Biosensors
This is a multidisciplinary effort to integrate microelectromechanical system technology and molecular biology in the development of a system for ultrasensitive detection uropathogen-specific nucleic acids within a time frame that would enable point-of-care diagnosis and treatment.
Role: CoPI
